e-box (VILBER GmbH)
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e-box
E Box, supplied by VILBER GmbH, used in various techniques. Bioz Stars score: 98/100, based on 3365 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gel+documentation+system/E-BOX/custom%40e-box-1%4010%2E1016%2Fj%2Enexres%2E2026%2E102105
Average 98 stars, based on 3365 article reviews
E Box, supplied by VILBER GmbH, used in various techniques. Bioz Stars score: 98/100, based on 3365 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gel+documentation+system/E-BOX/custom%40e-box-1%4010%2E1016%2Fj%2Enexres%2E2026%2E102105
Average 98 stars, based on 3365 article reviews
e-box - by Bioz Stars,
2026-09
98/100 stars
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Polymerase Chain Reaction:Article Title: Genomic Organization of the Newly Discovered Cassava Congo Cheravirus Reveals a Unique Maf/HAM1 Motif in the C-Terminal Region of the RNA1 Polyprotein and Suggests the Presence of Two Protein Domains Upstream of the Putative Helicase Domain. Article Snippet: A 10 μL PCR mixture containing 5.8 μL nuclease-free water, 2 μL PCR buffer (5X), 0.40 μL MgCl2 (50 mM), 0.20 μL dNTPs (10 mM), 0.20 μL of each primer (10 mM), 0.4 μL Mango Taq DNA polymerase (Meridian Bioscience®, Luckenwalde/Berlin, Germany), and 1.0 μL of cDNA. .. PCR products were analyzed by electrophoresis in TAE buffer (1X) on a 1% agarose gel stained with Gel red® (Biotium, Fremont, CA, USA), visualized under UV light, and photographed using a Article Title: DNA barcoding-based assessment of genetic variation in selected Southwest Nigerian medicinal Senna species (Caesalpinoideae: Fabaceae) Article Snippet: .. Gel electrophoresis After PCR amplification, 2 μL of PCR product was run on 1% agarose gel, stained with 1 μL of safe view Ethidium Bromide (EtBr) (10 mg/ml), and photographed using a Article Title: Nested PCR as a superior diagnostic method for Acanthamoeba keratitis compared to conventional techniques. Article Snippet: PCR amplification was carried out in a Peqlab peqSTAR, Thermal Cyclers (PEQ LAB, Germany) using the following thermal cycling conditions: the initial denaturing step of 5 min at 95 °C, followed by 35 cycles at 94 °C for 45 s, 60 °C for 45 s, 72 °C for 45 s, and final extension of 72 °C for 10 min. Acanthamoeba T4 genotype was used as a positive control, and a non-template control as a negative control. .. PCR amplifications were observed by electrophoresis of 4 μL PCR product aliquots in a 1.5% agarose gel containing safe stain, and amplicon bands were examined using the Vilber Lourmat Article Title: α-Thalassemia has no association with asymptomatic Plasmodium falciparum carriage in three ecological zones of Ghana. Article Snippet: .. PCR amplicons were resolved on a 2% agarose gel stained with ethidium bromide, electrophoresed at 120 V for 40 minutes, and visualized using Article Title: α-Thalassemia has no association with asymptomatic Plasmodium falciparum carriage in three ecological zones of Ghana Article Snippet: .. PCR amplicons were resolved on a 2% agarose gel stained with ethidium bromide, electrophoresed at 120 V for 40 min, and visualized using Article Title: Association of GSTM1 and GSTT1 Null Genotypes with Disease Severity and Serum Cytokine Levels in Hospitalized COVID-19 Patients Article Snippet: Thermal cycling was conducted in a thermocycler (Eppendorf Mastercycler Personal, Eppendorf, Hamburg, Germany) under the following conditions: initial denaturation at 95 ◦C for 5 minutes, followed by 30 amplification cycles consisting of denaturation at 94 ◦C for 30 seconds, primer annealing at 64 ◦C for 30 seconds, and extension at 72 ◦C for 45 seconds, with a final elongation step at 72 ◦C for 7 minutes. .. Following amplification, PCR products were separated by electrophoresis on a 2% agarose gel stained with ethidium bromide and visualized under ultraviolet light using a Electrophoresis:Article Title: Genomic Organization of the Newly Discovered Cassava Congo Cheravirus Reveals a Unique Maf/HAM1 Motif in the C-Terminal Region of the RNA1 Polyprotein and Suggests the Presence of Two Protein Domains Upstream of the Putative Helicase Domain. Article Snippet: A 10 μL PCR mixture containing 5.8 μL nuclease-free water, 2 μL PCR buffer (5X), 0.40 μL MgCl2 (50 mM), 0.20 μL dNTPs (10 mM), 0.20 μL of each primer (10 mM), 0.4 μL Mango Taq DNA polymerase (Meridian Bioscience®, Luckenwalde/Berlin, Germany), and 1.0 μL of cDNA. .. PCR products were analyzed by electrophoresis in TAE buffer (1X) on a 1% agarose gel stained with Gel red® (Biotium, Fremont, CA, USA), visualized under UV light, and photographed using a Article Title: Nested PCR as a superior diagnostic method for Acanthamoeba keratitis compared to conventional techniques. Article Snippet: PCR amplification was carried out in a Peqlab peqSTAR, Thermal Cyclers (PEQ LAB, Germany) using the following thermal cycling conditions: the initial denaturing step of 5 min at 95 °C, followed by 35 cycles at 94 °C for 45 s, 60 °C for 45 s, 72 °C for 45 s, and final extension of 72 °C for 10 min. Acanthamoeba T4 genotype was used as a positive control, and a non-template control as a negative control. .. PCR amplifications were observed by electrophoresis of 4 μL PCR product aliquots in a 1.5% agarose gel containing safe stain, and amplicon bands were examined using the Vilber Lourmat Article Title: Association of GSTM1 and GSTT1 Null Genotypes with Disease Severity and Serum Cytokine Levels in Hospitalized COVID-19 Patients Article Snippet: Thermal cycling was conducted in a thermocycler (Eppendorf Mastercycler Personal, Eppendorf, Hamburg, Germany) under the following conditions: initial denaturation at 95 ◦C for 5 minutes, followed by 30 amplification cycles consisting of denaturation at 94 ◦C for 30 seconds, primer annealing at 64 ◦C for 30 seconds, and extension at 72 ◦C for 45 seconds, with a final elongation step at 72 ◦C for 7 minutes. .. Following amplification, PCR products were separated by electrophoresis on a 2% agarose gel stained with ethidium bromide and visualized under ultraviolet light using a Agarose Gel Electrophoresis:Article Title: Genomic Organization of the Newly Discovered Cassava Congo Cheravirus Reveals a Unique Maf/HAM1 Motif in the C-Terminal Region of the RNA1 Polyprotein and Suggests the Presence of Two Protein Domains Upstream of the Putative Helicase Domain. Article Snippet: A 10 μL PCR mixture containing 5.8 μL nuclease-free water, 2 μL PCR buffer (5X), 0.40 μL MgCl2 (50 mM), 0.20 μL dNTPs (10 mM), 0.20 μL of each primer (10 mM), 0.4 μL Mango Taq DNA polymerase (Meridian Bioscience®, Luckenwalde/Berlin, Germany), and 1.0 μL of cDNA. .. PCR products were analyzed by electrophoresis in TAE buffer (1X) on a 1% agarose gel stained with Gel red® (Biotium, Fremont, CA, USA), visualized under UV light, and photographed using a Article Title: Genomic and immunoinformatic characterization of canine adenovirus type 1 in parvovirus-infected dogs: A comprehensive study from northeast India. Article Snippet: Canine adenovirus type 1 (CAdV-1), the causative agent of infectious canine hepatitis (ICH), a fatal disease affecting domestic and wild canids, yet its prevalence and molecular characteristics remain underexplored in India’s north eastern region (NER).. This study presents the first comprehensive genomic and immunoinformatic analysis of CAdV-1 in dogs in the region.. Out of 208 canine parvovirus type-2 (CPV-2) positive fecal samples, 36 (17.30%) tested positive for CAdV-1 by PCR. Article Title: DNA barcoding-based assessment of genetic variation in selected Southwest Nigerian medicinal Senna species (Caesalpinoideae: Fabaceae) Article Snippet: .. Gel electrophoresis After PCR amplification, 2 μL of PCR product was run on 1% agarose gel, stained with 1 μL of safe view Ethidium Bromide (EtBr) (10 mg/ml), and photographed using a Article Title: Nested PCR as a superior diagnostic method for Acanthamoeba keratitis compared to conventional techniques. Article Snippet: PCR amplification was carried out in a Peqlab peqSTAR, Thermal Cyclers (PEQ LAB, Germany) using the following thermal cycling conditions: the initial denaturing step of 5 min at 95 °C, followed by 35 cycles at 94 °C for 45 s, 60 °C for 45 s, 72 °C for 45 s, and final extension of 72 °C for 10 min. Acanthamoeba T4 genotype was used as a positive control, and a non-template control as a negative control. .. PCR amplifications were observed by electrophoresis of 4 μL PCR product aliquots in a 1.5% agarose gel containing safe stain, and amplicon bands were examined using the Vilber Lourmat Article Title: α-Thalassemia has no association with asymptomatic Plasmodium falciparum carriage in three ecological zones of Ghana. Article Snippet: .. PCR amplicons were resolved on a 2% agarose gel stained with ethidium bromide, electrophoresed at 120 V for 40 minutes, and visualized using Article Title: α-Thalassemia has no association with asymptomatic Plasmodium falciparum carriage in three ecological zones of Ghana Article Snippet: .. PCR amplicons were resolved on a 2% agarose gel stained with ethidium bromide, electrophoresed at 120 V for 40 min, and visualized using Article Title: Association of GSTM1 and GSTT1 Null Genotypes with Disease Severity and Serum Cytokine Levels in Hospitalized COVID-19 Patients Article Snippet: Thermal cycling was conducted in a thermocycler (Eppendorf Mastercycler Personal, Eppendorf, Hamburg, Germany) under the following conditions: initial denaturation at 95 ◦C for 5 minutes, followed by 30 amplification cycles consisting of denaturation at 94 ◦C for 30 seconds, primer annealing at 64 ◦C for 30 seconds, and extension at 72 ◦C for 45 seconds, with a final elongation step at 72 ◦C for 7 minutes. .. Following amplification, PCR products were separated by electrophoresis on a 2% agarose gel stained with ethidium bromide and visualized under ultraviolet light using a Staining:Article Title: Genomic Organization of the Newly Discovered Cassava Congo Cheravirus Reveals a Unique Maf/HAM1 Motif in the C-Terminal Region of the RNA1 Polyprotein and Suggests the Presence of Two Protein Domains Upstream of the Putative Helicase Domain. Article Snippet: A 10 μL PCR mixture containing 5.8 μL nuclease-free water, 2 μL PCR buffer (5X), 0.40 μL MgCl2 (50 mM), 0.20 μL dNTPs (10 mM), 0.20 μL of each primer (10 mM), 0.4 μL Mango Taq DNA polymerase (Meridian Bioscience®, Luckenwalde/Berlin, Germany), and 1.0 μL of cDNA. .. PCR products were analyzed by electrophoresis in TAE buffer (1X) on a 1% agarose gel stained with Gel red® (Biotium, Fremont, CA, USA), visualized under UV light, and photographed using a Article Title: DNA barcoding-based assessment of genetic variation in selected Southwest Nigerian medicinal Senna species (Caesalpinoideae: Fabaceae) Article Snippet: .. Gel electrophoresis After PCR amplification, 2 μL of PCR product was run on 1% agarose gel, stained with 1 μL of safe view Ethidium Bromide (EtBr) (10 mg/ml), and photographed using a Article Title: Nested PCR as a superior diagnostic method for Acanthamoeba keratitis compared to conventional techniques. Article Snippet: PCR amplification was carried out in a Peqlab peqSTAR, Thermal Cyclers (PEQ LAB, Germany) using the following thermal cycling conditions: the initial denaturing step of 5 min at 95 °C, followed by 35 cycles at 94 °C for 45 s, 60 °C for 45 s, 72 °C for 45 s, and final extension of 72 °C for 10 min. Acanthamoeba T4 genotype was used as a positive control, and a non-template control as a negative control. .. PCR amplifications were observed by electrophoresis of 4 μL PCR product aliquots in a 1.5% agarose gel containing safe stain, and amplicon bands were examined using the Vilber Lourmat Article Title: α-Thalassemia has no association with asymptomatic Plasmodium falciparum carriage in three ecological zones of Ghana. Article Snippet: .. PCR amplicons were resolved on a 2% agarose gel stained with ethidium bromide, electrophoresed at 120 V for 40 minutes, and visualized using Article Title: α-Thalassemia has no association with asymptomatic Plasmodium falciparum carriage in three ecological zones of Ghana Article Snippet: .. PCR amplicons were resolved on a 2% agarose gel stained with ethidium bromide, electrophoresed at 120 V for 40 min, and visualized using Article Title: Association of GSTM1 and GSTT1 Null Genotypes with Disease Severity and Serum Cytokine Levels in Hospitalized COVID-19 Patients Article Snippet: Thermal cycling was conducted in a thermocycler (Eppendorf Mastercycler Personal, Eppendorf, Hamburg, Germany) under the following conditions: initial denaturation at 95 ◦C for 5 minutes, followed by 30 amplification cycles consisting of denaturation at 94 ◦C for 30 seconds, primer annealing at 64 ◦C for 30 seconds, and extension at 72 ◦C for 45 seconds, with a final elongation step at 72 ◦C for 7 minutes. .. Following amplification, PCR products were separated by electrophoresis on a 2% agarose gel stained with ethidium bromide and visualized under ultraviolet light using a Marker:Article Title: Genomic and immunoinformatic characterization of canine adenovirus type 1 in parvovirus-infected dogs: A comprehensive study from northeast India. Article Snippet: Canine adenovirus type 1 (CAdV-1), the causative agent of infectious canine hepatitis (ICH), a fatal disease affecting domestic and wild canids, yet its prevalence and molecular characteristics remain underexplored in India’s north eastern region (NER).. This study presents the first comprehensive genomic and immunoinformatic analysis of CAdV-1 in dogs in the region.. Out of 208 canine parvovirus type-2 (CPV-2) positive fecal samples, 36 (17.30%) tested positive for CAdV-1 by PCR. Article Title: Molecular detection of Helicobacter pylori in saliva of Sri Lankan adults with periodontitis, gastritis or both conditions. Article Snippet: .. A 100 bp DNA ladder (Promega, USA) was used as a molecular size marker, and the gels were visualized with a Nucleic Acid Electrophoresis:Article Title: DNA barcoding-based assessment of genetic variation in selected Southwest Nigerian medicinal Senna species (Caesalpinoideae: Fabaceae) Article Snippet: .. Gel electrophoresis After PCR amplification, 2 μL of PCR product was run on 1% agarose gel, stained with 1 μL of safe view Ethidium Bromide (EtBr) (10 mg/ml), and photographed using a Amplification:Article Title: DNA barcoding-based assessment of genetic variation in selected Southwest Nigerian medicinal Senna species (Caesalpinoideae: Fabaceae) Article Snippet: .. Gel electrophoresis After PCR amplification, 2 μL of PCR product was run on 1% agarose gel, stained with 1 μL of safe view Ethidium Bromide (EtBr) (10 mg/ml), and photographed using a Article Title: Nested PCR as a superior diagnostic method for Acanthamoeba keratitis compared to conventional techniques. Article Snippet: PCR amplification was carried out in a Peqlab peqSTAR, Thermal Cyclers (PEQ LAB, Germany) using the following thermal cycling conditions: the initial denaturing step of 5 min at 95 °C, followed by 35 cycles at 94 °C for 45 s, 60 °C for 45 s, 72 °C for 45 s, and final extension of 72 °C for 10 min. Acanthamoeba T4 genotype was used as a positive control, and a non-template control as a negative control. .. PCR amplifications were observed by electrophoresis of 4 μL PCR product aliquots in a 1.5% agarose gel containing safe stain, and amplicon bands were examined using the Vilber Lourmat Article Title: Association of GSTM1 and GSTT1 Null Genotypes with Disease Severity and Serum Cytokine Levels in Hospitalized COVID-19 Patients Article Snippet: Thermal cycling was conducted in a thermocycler (Eppendorf Mastercycler Personal, Eppendorf, Hamburg, Germany) under the following conditions: initial denaturation at 95 ◦C for 5 minutes, followed by 30 amplification cycles consisting of denaturation at 94 ◦C for 30 seconds, primer annealing at 64 ◦C for 30 seconds, and extension at 72 ◦C for 45 seconds, with a final elongation step at 72 ◦C for 7 minutes. .. Following amplification, PCR products were separated by electrophoresis on a 2% agarose gel stained with ethidium bromide and visualized under ultraviolet light using a |